I thought I was going to have some down time before I posted again, but I ran into this interesting isolation paper of forazoline A that is worth a gander from Tim Bugni’s lab in the University of Wisconsin. They used a 15N-13C HMQC technique to determine which carbon was bonded to which nitrogen, which is apparently the first time this technique was used for a natural product. Looks like a pretty large natural product (m/z 901.4205). They have an interesting looking 13C NMR spectrum of the natural product (13C-labeled) that have splitting patterns on basically all of the carbons of the natural product (and thus looks like a proton NMR).
Archive for the ‘New Setups’ Category
Posted by naturalproductman on September 10, 2014
Posted by naturalproductman on January 20, 2014
Burkhard Luy and co-workers at Karlsruher Institut für Technologie in Germany have reported in JACS on a quick HSQC NMR method.
Posted by naturalproductman on September 6, 2013
Gareth Morris and co-workers at the University of Manchester and beyond have reported in ACIE on enhancing the resolution of HSQC contours. I imagine this would be useful when carbon chemical shifts and proton chemical shifts are jumbled together.
Posted by naturalproductman on June 5, 2013
Klaus Zangger and co-worker at Karl-Franzens Universität Graz have reported in ACIE on a method to make your protons all look like singlets in your proton NMR.
Posted by naturalproductman on May 14, 2013
I have had this silly problem in trying to get rid of triphenylphosphine oxide from my reaction mixture. It’s stuck with my final product and can be a pain. Some tricks I’ve seen are the use of dissolving mixture (O=PPh3 and product) in ether and run through short pad of silica. This would be great if my product weren’t so polar – instead my product does not elute with ether. Another trick was from this Lipshutz paper in 2003 where they used Merrifield resin to scavenge the phosphine.
Posted by naturalproductman on April 17, 2012
Ion Ghiviriga at the University of Florida has reported in JOC on the use of NOESY1D to determine the stereochemistry of secondary alcohols by converting the alcohol to an MPA ester.
Posted by naturalproductman on June 6, 2011
Frank Schroeder and co-workers at Cornell have reported in JACS on the isolation of gli dependent metabolites using differential analysis by 2D NMR spectroscopy (DANS).