Naturalproductman’s Blog

Hey, is that a natural product?!

Archive for the ‘Enzymes’ Category

Going from a diol to a tetrahydropyran in salinomycin

Posted by naturalproductman on September 25, 2015

Peter Leadlay and co-workers from the University of Cambridge have reported in ACIE on the enzyme that makes the tetrahydropyran ring in salinomycin. The tetrahydropyran is proposed to come from a diol where one of the hydroxy groups is in the beta-position to a ketone and subsequently dehydrates to form an enone. The enone undergoes an intramolecular Michael reaction to form the tetrahydropyran. However, when the reaction is done in vitro with the purified enzyme, they don’t see the enone intermediate, so I wonder if it is a processive reaction; in other words, the enone forms in the enzyme active site without being released and cyclizes to the tetrahydropyran.


ACIE paper

Posted in Biosynthesis, Chemical Biology, Enzymes, Mechanistic, Polyketides, Tetrahydropyran | Leave a Comment »

Enzyme from bacteria breaks down nicotine

Posted by naturalproductman on August 7, 2015

I’m sure this is not what the authors meant by battling nicotine addiction, but it made me laugh to think that a bacterial enzyme will be responsible to fight nicotine addiction – and immediately I thought that are we going to spray the smokers with this bacteria that has this enzyme?

JACS paper

Posted in Enzymes | Leave a Comment »

Welcome to the future

Posted by naturalproductman on August 7, 2015

I read the title of this paper and immediately thought of David Baker’s work. I guess we are getting closer to having a logical approach available to design our own protein catalysts.
JACS paper

Posted in Computational, Enzymes, Methodology | 2 Comments »

Cyclopropanation with iron(III) phthalocyanine

Posted by naturalproductman on May 2, 2015

Emily Balskus and colleague have reported in ACIE on an iron(III) phthalocyanine catalyst that can perform cyclopropanation reactions. Reminds me of the P450 version that was published a few years back.

ACIE paper

Posted in Enzymes, Iron, Methodology, Transition Metal | Leave a Comment »

Penitrem biosynthesis

Posted by naturalproductman on April 12, 2015

Hideaki Oikawa and co-workers at Hokkaido University have reported in ACIE on the enzymatic biosynthesis of penitrem.


ACIE paper

Posted in Biosynthesis, Cyclobutanes, Diterpene, Enzymes, Indoles | Leave a Comment »

Corvol ethers A and B biosynthesis

Posted by naturalproductman on March 25, 2015

Jeroen Dickschat and co-workers at Technische Universität Braunschweig have reported in ACIE on some terpene biosynthesis by characterizing a sesquiterpene cyclase enzyme.

ACIE paper

Posted in Biosynthesis, Enzymes, Sesquiterpenes | Leave a Comment »

Terpene cyclization involving carbons switching

Posted by naturalproductman on March 5, 2015

Tomohisa Kuzuyama and co-workers at the University of Tokyo have reported in ACIE on an mechanistically interesting terpene cyclization involving two carbons switching.


ACIE paper

Posted in Biosynthesis, Enzymes, Mechanistic, Proteins | Leave a Comment »

Cycloclavine biosynthesis

Posted by naturalproductman on March 5, 2015

Sarah O’Connor and co-workers from the John Innes Centre in the UK have reported in ACIE on the biosynthesis of cycloclavine.


ACIE paper

Posted in Alkaloids, Biosynthesis, Enzymatic, Enzymes, Methodology | Leave a Comment »


Posted by naturalproductman on February 22, 2013

Hayamitsu Adachi and co-workers from BIKAKEN have reported in JNP on a novel VEGF receptor tyrosine kinase inhibitor from bacteria. The only problem I see with this compound – from a toxicologist’s perspective – is that this is a quinone, and thus a very reactive electrophile. If this kind of functional group is used as a drug, who knows what other things it will react with in the body – be it DNA, proteins, etc…

JNP paper

Posted in Alkaloids, Enzymes, Protein Targets, Proteins | Leave a Comment »

Not just isolating small molecules anymore…

Posted by naturalproductman on January 17, 2013

Hideaki Oikawa and co-workers from Hokkaido University have reported in Organic Letters on the isolation of an enzyme that was known to produce some natural products. They expressed and purified the enzyme, incubated the enzyme in the presence of substrates:  geranyl diphosphate (GPP) and isopentenyl diphosphate (IPP), and examined the products.

sesterterpene synthase

OL paper

Posted in Chemical Biology, Enzymes, Methodology, Proteins, Sesterterpenes | Leave a Comment »


Get every new post delivered to your Inbox.

Join 81 other followers